References:
- Kobori N, Waymire J.C., Haycock JW, Clifton GL, Dash PK. Modulation of tyrosine hydroxylase phosphorylation and activity by glial cell line-derived neurotrophic factor. J. Biol. Chem. 279: 2182-2191, 2004
- Roe, D. and Craviso, G.L. and Waymire, J.C. Nicotinic stimulation modulates tyrosine hydroxylase mRNA half-life and protein binding to the 3’UTR in a manner that requires transcription Mol. Brain Res.. 120 91-102, 2004.
- Waymire, J.C., Activity-dependent regulation of neurotransmitter synthesis. Encyclopedia of Learning and Memory, J.W. Byrne (Ed.), Macmillan, New York, pp 1-5, 2002
- Waymire, J.C. and Haycock, J.W. Lack of regulation of aromatic L-amino acid decarboxylase in intact bovine chromaffin cells. J. Neurochem. 81: 589-593, 2002.
- Perez, R.G., Waymire, J. C., Slagel, S.L., Glessner, A.A., Liu, J.J., and Zigmond, M.J. A role for alpha synuclein in the regulation of dopamine biosynthesis. J. Neuroscience 22, 3090-3099, 2002.
Jack C. Waymire, Ph.D.
Professor
UTHSC-Medical School, (713) 500 - 5620
Jack.C.Waymire@uth.tmc.edu
Plasticity of neurotransmitter biosynthesis
The long-range objective of research in Dr. Waymire's laboratory is to determine the cellular and molecular changes required to establish catecholamine neurotransmitter secretion from adult and various stem cell lines. The goals of this work is to establish the underlying molecular and cellular processes responsible for the catecholamine phenotype and modulation of catecholamine biosynthesis and secretion within these cells. His laboratory accomplishes this studies to understand how tyrosine hydroxylase, the enzyme that regulates catecholamine biosynthesis, and several other catecholamine-specific proteins are regulated. His laboratory is currently working to understand: 1) the role of the phosphorylation 2) the role of the transcription and 3) the role of mRNA stabilization in modulating these catecholamine specific proteins.
A tutorial in Dr. Waymire's laboratory will provide experience in protein purification, HPLC peptide purification, protein phosphorylation, immunoblotting, mRNA analysis, in situ protein expression, mutagenesis, enzyme assays, and primary tissue culture.
