Zeiss 510 Meta Confocal Laser Scanning Microscope

The Zeiss 510 Meta is a high resolution microscope capable of Z sectioning and 3D reconstruction, capturing time series for following cellular events, fluorophore emission fingerprinting, determining co-localization of multiple proteins and/or dyes, spectral analysis FRET and FRAP.

Key features include:

6 laser lines

• Tunable Argon 458/477/488/514nm at 30 mW
• HeNe 543nm at 1 mW
• HeNe 633nm at 3 mW

2 PMTs (photomultiplier tubes)

Meta detector (polychromatic detector, can also be used as third PMT)

Axiovert 200 Wide field fluorescence microscope

Objectives

• 10X Plan-Neo/0.3 NA
• 20X Plan-Apo/0.75 NA
• 40X Plan-Neo/1.3 NA Oil with DIC capability
• 63X Plan-Apo/1.4 NA Oil

Accepts microscope slides and 35-100mm cell culture dishes (use 1.5 oz coverslips)